Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K336101

Designed by: Yuki Shimahara   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)

p(tetR)+α-amylase+luxR+lux reciever+ccdB

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

<Lux reciever> α-amylase and luxR are genarating by p(tetR). With Luxl protein, ccdB protein is genarating, and it leads cell death. After its death, α-amylase is released to out of the E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1503
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1503
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1503
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1720
    Illegal BsaI site found at 2537
    Illegal BsaI.rc site found at 2238


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Parameters
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